Proteomic Services
Our Proteomic Services consists of a wide range of analytical techniques to support your protein investigations. From basic protein assays (see Basic Services) to advanced mass spectrometry, CDI provides solutions that generate the data quality needed within an established budget. Mass spectrometry (MS) is an analytical technique used to measure the mass-to-charge (m/z) ratio of ions for qualitative and quantitative determinations. In the field of proteomics, it is commonly employed to determine intact mass measurements, protein identifications, and structural characteristics.
High Resolution (Definition) Mass Spectrometry
High Resolution Mass Spectrometry Analysis [AP-ILC-HRT]
This advanced proteomic service couples an ultra-performance liquid chromatograph to a high resolution mass spectrometer for the efficient separation and characterization of the protein composition of a sample. The data is processed to provide a list of proteins and their respective intact mass measurements. Relative and absolute quantification may also be performed. The detail of the final deliverables should be established prior to the commencement of work.
High Resolution Protein Identification [AP-ILC-PID]
Same as AP-ILC-HRT but utilizes a tandem mass spectrometry (tMS) approach to provide additional information about each ion detected. Once an ion (precursor ion) is isolated, it undergoes a unique fragmentation resulting in fragment ions (products ion) that help to elucidate the ion's characteristics or primary structure. In conventional "bottom-up" proteomic evaluations, a protein sample or mix is processed and digested with an endopeptidase, e.g. trypsin, to provide enzyme products that are separated, fragmented, and characterized. This data is processed by an algorithm to determine the proteins identified. The data deliverables will provide the false discovery rate (FDR), accession number, score, and coverage for proteins characterized in the databank repository.
NOTE: Available enzymes include trypsin, chymotrypsin, elastase, Lys-C, Glu-C, Asn-N, thermolysis, and pepsin. Trypsin is the default enzyme used for proteomic assays. If an alternative enzyme is requested, client can choose a substitute from the list above at no additional cost. However, if multiple enzymes are requested, please see AP-ILC-PPF.
High Resolution Protein Profiling [AP-ILC-PPF]
Same as AP-ILC-PID but utilizes a series of three enzymes to increase the number and confidence of protein identifications in the sample. This approach provides greater coverage and the identification of post translational-modifications with their respective loci, e.g. a phosphorylation, isomerization, glycosylation, etc. The report will also include a list of fragment peptides that were sequenced but did not match a protein in the databank repository along with their respective ALC score and PTM.
NOTE: Available enzymes include trypsin, chymotrypsin, elastase, Lys-C, Glu-C, Asn-N, thermolysin, and pepsin. Trypsin, chymotrypsin, and Asn-N are the default enzyme used for this proteomic assay. If alternative enzymes are requested other than trypsin, client can choose two substitutes from the list above at no additional cost.
High Resolution Protein de novo Analysis [AP-ILC-DNV]
This advanced proteomic service couples an ultra-performance liquid chromatograph to a high resolution tandem mass spectrometer for the elucidation of the complete primary structure of a protein. This will include the confirmed amino acid sequence, all post-translational modifications, and cysteine bridge configuration if present. Relative and absolute quantification may also be performed. The detail of the final deliverables should be established prior to the commencement of work.
NOTE: This service is only for proteins less than 50 residues. If there is greater than 50 residues in the sequence, please contact for more details.
Amino Acid Analysis
Amino Acid Analysis [AP-LTM-AAA]
Amino acids are the building blocks of proteins and are essential molecules in many biological processes. Whether you are performing nutrient analysis, amino acid composition of a protein, or just need to quantitate the presence of specific amnio acids in biological fluids, CDI offers an accurate quantification method utilizing an UPLC-tandem MS approach. No matter how complex the matrix, a validated method for absolute or relative quantification can be developed as per your application.
NOTE: This service is for amino acids beyond the common 20 and can be utilized for a significantly larger list, please contact CDI for more details.
Solid Phase Peptide Synthesis
Peptide Synthesis [BP-SPS-FMC & BP-SPS-BOC]
Peptides are small proteins that serve a variety of biochemical functions. CDI can purify peptides from a natural source, e.g. venom, or can synthesize a sequence provided by the client. Utilizing solid phase peptide synthesis, CDI produces high purity products with a variety of modifications. Please see our Synthetic Products for more details in choosing a service that's right for your project.