Our Research
Beyond the services offered to our clientele, CDI is committed to the study of venomics to elucidate the pharmaceutical potential of toxins given their biological activity. We work with a large consortium of venom investigators at a variety of analytical levels to support the development of libraries that can be examined for their endless possibilities in discovery. Below are a few examples of investigations underway to study the subtle post translational modifications that confer unique activity to a primary structure.
Aspartic Acid Isomerization Characterized by High Definition Mass Spectrometry Significantly Alters the Bioactivity of a Novel Toxin from Poecilotheria. Toxins (2020)
Stephen R Johnson (1)(2) & Hillary G Rikli (3)
(1) Carbon Dynamics Institute, LLC (2) Chemistry Department, University of Illinois Springfield, (3) College of Liberal Arts & Sciences, University of Illinois Springfield
Research in toxinology has created a pharmacological paradox. With an estimated 220,000 venomous animals worldwide, the study of peptidyl toxins provides a vast number of effector molecules. However, due to the complexity of the protein-protein interactions, there are fewer than ten venom-derived molecules on the market. Structural characterization and identification of post-translational modifications are essential to develop biological lead structures into pharmaceuticals. Utilizing advancements in mass spectrometry, we have created a high definition approach that fuses conventional high-resolution MS-MS with ion mobility spectrometry (HDMSE) to elucidate these primary structure characteristics. We investigated venom from ten species of "tiger" spider (Genus: Poecilotheria) and discovered they contain isobaric conformers originating from non-enzymatic Asp isomerization. One conformer pair conserved in five of ten examined, denominated PcaTX-1a and PcaTX-1b,
was found to be a 36-residue peptide with a cysteine knot, an amidated C-terminus, and isoAsp33Asp substitution. Although the isomerization of Asp has been implicated in many pathologies, this is the first characterization of Asp isomerization in a toxin and demonstrates the isomerized product's diminished physiological effects. This study establishes the value of a HDMSE approach to toxin screening and characterization.
A reexamination of poneratoxin from the venom of the bullet ant Paraponera clavata. Peptides (2017)
Stephen R Johnson(1)(2)(5), Hillary G Rikli(1)(2), Justin O Schmidt(3), M Steven Evans(4)
(1) Department of Biology, University of Illinois Springfield, (2) Department of Chemistry, University of Illinois Springfield, (3) Southwestern Biological Institute, (4) Department of Neurology, University of Louisville, (5) Carbon Dynamics Institute, LLC
In 1991, Piek et al. described a voltage-gated sodium channel (VGSC) modifier from “bullet ant” (Paraponera clavata) venom they called poneratoxin (PoTx). Using UV chromatography and Edman degradation they showed two “identical peptides” of 25 residues. We reinvestigated PoTx using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-TMS). De novo sequencing showed the two peptides were actually structurally different peptides: the originally described PoTx and a glycyl pro-peptide (glycyl-PoTx) that lacks C-terminus amidation. We examined P. clavata venom from different geographical locations and discovered two additional PoTx analogs: an A23E substitution analog and a D22N; A23V substitutions analog. We tested PoTx and these three natural analogs on the mammalian sensory voltage-gated sodium channel, Nav1.7, using whole cell voltage-clamp.
PoTx and each analog induced slowly activating currents in response to small depolarizing steps and sustained currents due to blockade of channel inactivation, similar to that described previously in skeletal muscle. Glycyl-PoTx had the same potency and efficacy as PoTx. A23E PoTx, with a decrease in both C-terminal net positive charge and hydrophobicity, had an eight-fold reduction in potency compared to PoTx. In contrast, the D22N; A23V PoTx, with an increase in both C-terminal net positive charge and hydrophobicity, had a nearly five-fold increase in potency compared to PoTx. We found that changes in PoTx C-terminus caused a significant change in PoTx potency.